Peak intensity of light emission occurs at 440 nm. Reaction of the substrate with HRP enzyme generates rapid luminescence of the highest intensity within seconds. With this stability, the working solution can be prepared and stored for later use. The working solution is stable at room temperature for at least one week and at 2-8° C for at least one month. Similar to Lumigen ECL Extra, Lumigen ECL Ultra has excellent stability. Sustained signal duration - over several hours allows convenient imaging.Temperature insensitive - analytical results are insensitive to temperatures from 22° C - 35° C reducing the need for precise temperature control Overview Specifications Related Documents Order Table Product Support Amersham ECL Prime is a highly sensitive chemiluminescent detection reagent and is characterized by extremely stable signal emission. Western blot detection kit Chemiluminescent substrate based on luminol High sensitivity Detection of antigens with very low concentrations Long signal. When performing Western blotting, use a sufficient amount of blocking buffer, washing buffer, and chemiluminescent substrate.Reduced antibody usage - more than 10-fold reduction in the usage of expensive antibody conjugates Below is my protocol: - Blocking RT, 2 hours with 5 BSA - Primary antibody p-Akt (1:2000), overnight, RT - Secondary antibody Anti rabbit with HRP (1:3000), 1 hour 30 minutes, RT.Highest sensitivity - mid to low femtogram.Very rapid onset of chemiluminescence - images can be recorded in seconds.3.2.1 Mix an equal volume of EZ-ECL Solution A and EZ-ECL Solution B to give sufficient. Lumigen ECL Ultra (TMA-6) is a proprietary acridan based chemiluminescent substrate for ultra-sensitive detection of Western blotted and ELISA captured proteins that are bound to horseradish peroxidase (HRP) labeled antibodies. MCE Ultra High Sensitivity ECL Kit is an ultra-sensitive, luminol-based enhanced chemiluminescent substrate for detecting horseradish peroxidase (HRP). Protocol for Western Blotting and Chemiluminescence Detection.
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